Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1

Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1

Blog Article

Multiheme cytochromes play a central role in extracellular electron transfer, a process that allows ladies pants microorganisms to sustain their metabolism with external electron acceptors or donors.In Shewanella oneidensis MR-1, the decaheme cytochromes OmcA and MtrC show functional specificity for interaction with soluble and insoluble redox partners.In this work, the capacity of extracellular electron transfer by mutant variants of S.oneidensis MR-1 OmcA was investigated.The results show that amino acid mutations can affect protein stability and alter the redox properties of the protein, without affecting the ability to perform extracellular electron transfer to methyl orange dye or a poised electrode.

The results also show that there is a good correlation between the reduction of the dye and the current generated at the electrode for most but not SHOWER PURIFIER #360 all mutants.This observation opens the door for investigations of the molecular mechanisms of interaction with different electron acceptors to tailor these surface exposed cytochromes towards specific bio-based applications.